Information on making liposomes from Avanti lipids web site
We apply a variety of methods to the study of endocytosis. Some of our favorites include:
X-ray crystallography In collaboration with the laboratory of Phil Evans, the group has solved the structures of a number of endocytic proteins and domains.
See the structures of some endocytic proteins
Isothermal titration calorimetry to quantitate protein-protein interactions
Lipid Monolayer assay to reconstitute early steps of endocytosis
Overexpression of endocytic proteins in COS cells to test the effects of mutations in vivo
Liposome/lipid nanotube sedimentation assays to test lipid binding and specificity
(also immunoprecipitations, cloning, kinase assays, etc.)
Eva Schmid's methods from thesis: HeLa cell extracts, Dictyostelium methods, Yeast-2-hybrid and Surface Plasmon Resonance.
Transferrin endocytosis assay for cultured cells