Developed by Matthew Higgins, Brian Peter, Barbara Pearse, and Harvey McMahon
A basic schematic of the technique is below.
A gallery of images and detailed protocol are also available.
Add PIP2-containing lipid mix in drop of chlorof
orm/methanol to well of buffer
Monolayer forms as solvent evaporates
Place carbon-coated EM grid on monolayer
Inject proteins underneath monolayer
Incubate at room temperature; PIP2
Remove EM grid, stain with uranyl acetate, and visualize by EM
See more examples of monolayer EMs
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