|Myc-EpsinR with endogenous TGN46 and AP1 in COS cells
In the image to the left there are 2 cells transfected with Myc-epsinR and 3 cells not transfected. In the left cells endogenous AP1 is finely punctate and co-localises
with the more tubular stain of endogenous TGN46. In the right transfected cell both the perinuclear AP1 stain and TGN46 stain are less compact and thus the co-localisation with epsinR is easier to visualise. We see that many of the perinuclear epsinR puncta co-stain for TGN46 and the AP1 complex. In non transfected cells overlapping TGN46 and AP1 are pink.
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A TGN localisation for a proportion of the epsinR is supported by the co-fractionation with TGN markers during organelle purification and the inhibitory effect of overexpression on trafficking of cathepsinD (see paper).