This material is supplemental to Ford et al., "Curvature of clathrin coated pits driven by epsin" Nature, 419: 361-366 (.pdf).

Supplementary Table 2 Calorimetric parameters.

KD

(mM)

KA

(M-1)

DH

(kcal mol-1)

TDS

(kcal mol-1)

Epsin1 ENTH WT

Ins(1,4)P3

No binding

Ins(1,5)P3

No binding

Ins(1,3,5)P3

İİ 120

8.6 x 103

-11.5

-6.4

Ins(1,4,5)P3

İİ 3.6

2.9 x 105

-20.0

-12.9

Ins(1,4,5)P3İİİİİİİİİİİİİİİİİİ 20 ƒC

İİ 11

9.7 x 104

-21.1

-14.4

Ins(1,4,5)P3İİİİİİİİİİİİİİİİİİ 25 ƒC

İİ 29

3.4 x 104

-21.7

-15.5

Ins(1,4,5)P3İİİİİİİİİİİİİİİİİİ 37 ƒC

İİ 32

3.1 x 104

-22.1

-15.9

Ins(1,3,4,5)P3

İİ 4.1

2.4 x 105

-17.7

-10.7

InsP6

İİ 0.55

1.8 x 106

-16.0

-7.9

diC8PtdIns(4,5)P2

İİ 0.84

1.2 x 106

-11.1

-3.2

Epsin1 ENTH L6Q

Ins(1,4,5)P3

İİ 3.0

3.3 x 105

-20.4

-13.2

diC8PtdIns(4,5)P2

İİ 2.2

4.6 x 105

-17.0

-9.6

Epsin1 ENTH L6E

Ins(1,4,5)P3

İİ 5.3

1.9 x 105

-21.4

-14.5

diC8PtdIns(4,5)P2

İİ 5.2

1.9 x 105

-15.0

-8.2

Epsin1 ENTH L6E + I13D

Ins(1,4,5)P3

İİ 27

3.7 x 104

-19.2

-13.3

Epsin1 ENTH R63L + H73L

Ins(1,4,5)P3

No binding

AP180 ANTH WT

Ins(1,4,5)P3

30

3.4 x 104

-4.8

1.1

InsP6İİİİİİİİİİİ (2 ANTH/InsP6)

5.1

2.2 x 105

-4.9

2.0

Link to other supplemental data

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