This material is supplemental to Peter et al., "BAR Domains as Sensors of Membrane Curvature: The Amphiphysin BAR Structure" Science Express Nov 26 (abstract); print version Jan 23, 2004.
Fig. S1. Analytical ultracentrifugation of Drosophila amphiphysin N-BAR
The figure shows a plot of apparent molecular weight (M w,app) against concentration (left), overlaying the individual cells, and also plots of the residuals for each cell against radius (right). In practice, the correction factor for M1 was close to 1.0, while the concentration correction was <0.05 absorbance units. The line plotted on the left figure was calculated with M1=40kDa and Kd=6microM, as derived from the fitting (see methods and .pdf file of analysis). The correspondence of the data with this line and, even more, the small and randomly distributed residuals for fitting the raw data, confirm that the Drosophila amphiphysin N-BAR reversibly dimerized under these conditions. Similar results were obtained for full-length arfaptin and centaurinbeta2 BAR+PH.
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